A Method for Estimating Numbers of Eggs of Meloidogyne spp. in Soil.
نویسندگان
چکیده
A procedure for extracting eggs of Meloidogyne spp. from soil was developed by modifying and combining certain existing techniques. Egg masses were elutriated from the soft, gelatinous matrices of the egg masses were dissolved, and the dispersed eggs were stained to facilitate counting. Data on egg population densities thus obtained facilitate the study of population dynamics of Meloidogyne spp. and the analysis of root-knot epidemics.
منابع مشابه
Water absorbent polymer AIDS in the infestation of field sites with meloidogyne eggs.
The root-knot nematode (Meloidogyne spp.) is one of the principal nematode pests of field and vegetable crops in the southern United States (6). Meloidogyne spp. are generally not uniformly distributed in naturally infested field sites, and sites frequently contain more than one species, thus complicating the evaluation of breeding lines for nematode resistance. To facilitate evaluation of nema...
متن کاملA technique for the collection of larvae of Meloidogyne spp. and a comparison of eggs and larvae as inocula.
T h e infectivity of second-stage larvae of Meloidogyne spp. is a function of soil temperature, aeration, moisture, texture, and density, as well as a funct ion of the distance the second-stage larvae have to travel pr ior to root penetra t ion (3, 4). Because eggs may require up to 2 weeks to hatch under greenhouse conditions, infection periods with larvae are normal ly shorter than those with...
متن کاملSuppression of Meloidogyne incognita and M. javanica by Pasteuria penetrans in Field Soil.
The role of Pasteuria penetrans in suppressing numbers of root-knot nematodes was investigated in a 7-year monocuhure of tobacco in a field naturally infested with a mixed population of Meloidogyne incognita race 1 and M. javanica. The suppressiveness of the soil was tested using four treatments: autoclaving (AC), microwaving (MW), air drying (DR), and untreated. The treated soil bioassays cons...
متن کاملOptimum Initial Inoculum Levels for Evaluation of Resistance in Tomato to Meloidogyne spp. at Two Different Soil Temperatures.
The effects of Meloidogyne incognita or M. javanica at five initial inoculum levels of 20, 100, 200, 1,000, and 2,000 eggs and infective juveniles per seedling on 'Floradade,' 'Nemarex,' 'Patriot,' and 'PI 129149-2(sib)-5' tomatoes maintained at 25 or 32.5 C were studied. The number of egg masses on roots of the susceptible cultivar Floradade was similar for both species of root-knot nematodes ...
متن کاملA Novel Technique for Infesting Field Sites with Encapsulated Eggs of Meloidogyne spp.
Eggs of Meloidogyne arenaria race 1 were encapsulated in calcium alginate for use as inoculum to infest peanut field plots. Some eggs within the capsules remained viable up to 10 weeks after preparation. A field site was successfully infested at peanut planting and (or) 6 weeks later. Dual applications of nematode inoculum (at planting and 6 weeks later) were superior to single applications (at...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of nematology
دوره 4 4 شماره
صفحات -
تاریخ انتشار 1972